Search results for "bakteriālais metabolisms"

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GRM2 tipa bakteriālo mikrokompartmentu daļiņu raksturojums

2020

Bakteriālie mikrokompartmenti (BMC) ir baktēriju organellas, kas iepako enzīmus proteīnu čaulā. Šī darba mērķis bija analizēt iespējas GRM2 tipa BMC iepakot heterologus enzīmus, kā arī apskatīt rekombinanti producētu BMC čaulas īpašības. Šajā darbā Klebsiella pneumoniae GRM2 tipa BMC čaulā tika testēta trīs Zymomonas mobilis etanolģenēzes enzīmu iepakošana kā starpnieku izmantojot natīvo CutC holīna liāzes enzīmu un SpyTag/SpyCatcher adhezīnu sistēmu. Veikta arī bakteriālo mikrokompartmentu čaulas analīze ar cryo-EM. Darba ietvaros veikts konstruktu dizains, proteīnu ekspresija, attīrīšana, un analīze ar TEM un cryo-EM. Rezultāti liecina par zemu iepakošanas efektivitāti, ja kā mediators iz…

Klebsiella pneumoniaebakteriālie mikrokompartmenticryo-EMbakteriālais metabolismsBioloģijabiotehnoloģija

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